| Group | Club/Team Name | Team ID | Head Coach |
| 446-9 | 515 Soccer Academy 515 Soccer Academy - G2016 |
0446-01AG16-0009 | Kevin Connell |
| 443-18 | Apex Soccer Academy Apex 9UG Reign |
0443-01AG16-0018 | Mark Gavin |
| 402-2 | Bondurant Soccer Club BSC G9 Academy Blue |
0402-01AG16-0002 | Tony Phillips |
| 429-12 | Dallas Center Grimes Soccer Club DCG Academy U9 Girls Red |
0429-01AG16-0012 | Jamie Patrick-merged |
| 441-28 | Des Moines Soccer Club DMSC 2016 Girls Blue |
0441-01AG16-0028 | Matt Lamale |
| 441-27 | Des Moines Soccer Club DMSC 2016 Girls Red |
0441-01AG16-0027 | Matt Lamale |
| 112-3 | FK envision FK envision U9 Girls |
0112-01AG16-0003 | Stevan Krickovic |
| 418-31 | J-Hawk Soccer Club Iowa United FC Academy 9UG Red |
0418-01AG16-0031 | Dustin Shandri |
| 418-18 | J-Hawk Soccer Club Iowa United FC Academy 9UG White |
0418-01AG16-0018 | Cole Meyers |
| 421-48 | Pleasant Hill Soccer Club PHSC GU9 Academy Orange |
0421-01AG16-0048 | Scott Yount |
| 422-24 | United Futbol Academy UFA DSM 2015/2016G |
0422-01AG15-0024 | n/a |